{"id":12511,"date":"2017-06-08T09:57:05","date_gmt":"2017-06-08T09:57:05","guid":{"rendered":"http:\/\/revoscience.com\/en\/?p=12511"},"modified":"2017-06-08T09:57:05","modified_gmt":"2017-06-08T09:57:05","slug":"hidden-order-dna-diffusion","status":"publish","type":"post","link":"https:\/\/www.revoscience.com\/en\/hidden-order-dna-diffusion\/","title":{"rendered":"The hidden order in DNA diffusion"},"content":{"rendered":"

The movement of DNA molecules seemingly explained by random motion conceals a more orderly march.<\/strong><\/em><\/span><\/p>\n

\"\"
A new single-molecule tracking method based on fluorescence molecular imaging revealed nonrandom motion of DNA molecules.
Credit : \u00a9 KAUST Anastasia Khrenova<\/figcaption><\/figure>\n

A different approach to analyzing the motion of diffusing molecules has helped overturn the long-held assumption that DNA molecules move in a haphazard way. KAUST researchers reveal for the first time that DNA molecules move not by random Brownian motion but by a nonrandom walk related to polymer dynamics in a way that conserves overall Brownian characteristics.<\/span><\/p>\n

\u201cBrownian motion is a process whereby molecules move randomly in a fluid by colliding with other molecules,\u201d explained Dr. Maged Serag, a postdoctoral researcher in Bioscience at KAUST. \u201cIn living cells, Brownian motion allows molecules to move rapidly and efficiently between cell organelles and interact with other molecules.\u201d<\/span><\/p>\n

For many decades, scientists have used a relatively simple test to determine whether molecular diffusion is Brownian: when the mean-square displacement (MSD) of a population of molecules increases linearly over time. In a uniform medium like pure water, this means that a drop of saline solution will expand at a rate that makes the MSD increase linearly with time. <\/span><\/p>\n

DNA conforms to this macroscale diffusion behavior, and so it has been assumed that its motion is Brownian like other molecules. However, it is also known that DNA, being a long polymer molecule, writhes spontaneously due to intramolecular forces.<\/span><\/p>\n

\u201cThe DNA molecule can be viewed as a semi-flexible chain,\u201d said Serag. \u201cIf we follow its motion at short timescales and in a space close to its size, we see worm-like motional behavior.\u201d<\/span><\/p>\n

Serag and colleague Associate Professor Satoshi Habuchi set out to see whether this writhing motion could affect the diffusion of DNA.<\/span><\/p>\n

\u201cDr. Serag came up with a unique idea to describe the motion of a molecule based on the probability of occupying lattice sites rather than by mean-square displacement,\u201d said Habuchi. \u201cMSD has been the standard method to detect deviation from Brownian motion, but it does not reveal any nonrandom motion for DNA molecules. By using this probabilistic approach instead, we were able to detect and quantify hidden nonrandom motion.\u201d<\/span><\/p>\n

By developing a new theoretical framework in which motion is modeled in a step-wise manner accounting for molecular flexing, DNA molecules were found to move nonrandomly with varied speed and molecular \u2018track\u2019 in a way that precisely conserved the Brownian linear MSD. <\/span><\/p>\n

\u201cThe most important result of this study is that we have demonstrated that a linear MSD does not always indicate underlying Brownian motion,\u201d explained Habuchi. \u201cWith this new theoretical framework, we can detect the nonrandom motion of single molecules that cannot be captured by conventional MSD analysis.\u201d<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

The movement of DNA molecules seemingly explained by random motion conceals a more orderly march. A different approach to analyzing the motion of diffusing molecules has helped overturn the long-held assumption that DNA molecules move in a haphazard way. KAUST researchers reveal for the first time that DNA molecules move not by random Brownian motion […]<\/p>\n","protected":false},"author":6,"featured_media":12512,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17],"tags":[],"class_list":["post-12511","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research"],"featured_image_urls":{"full":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"thumbnail":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647-150x150.jpg",150,150,true],"medium":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647-300x225.jpg",300,225,true],"medium_large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"1536x1536":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"2048x2048":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"ultp_layout_landscape_large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"ultp_layout_landscape":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"ultp_layout_portrait":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"ultp_layout_square":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"newspaper-x-single-post":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"newspaper-x-recent-post-big":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"newspaper-x-recent-post-list-image":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",87,65,false],"web-stories-poster-portrait":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",400,300,false],"web-stories-publisher-logo":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",96,72,false],"web-stories-thumbnail":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/06\/4647.jpg",150,113,false]},"author_info":{"info":["Amrita Tuladhar"]},"category_info":"Research<\/a>","tag_info":"Research","comment_count":"0","_links":{"self":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts\/12511","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/comments?post=12511"}],"version-history":[{"count":0,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts\/12511\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/media\/12512"}],"wp:attachment":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/media?parent=12511"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/categories?post=12511"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/tags?post=12511"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}