{"id":13534,"date":"2017-11-05T07:57:06","date_gmt":"2017-11-05T07:57:06","guid":{"rendered":"https:\/\/www.revoscience.com\/en\/?p=13534"},"modified":"2017-11-05T07:57:06","modified_gmt":"2017-11-05T07:57:06","slug":"dioxane-chomping-microbe-helpful-gene","status":"publish","type":"post","link":"https:\/\/www.revoscience.com\/en\/dioxane-chomping-microbe-helpful-gene\/","title":{"rendered":"Dioxane-chomping microbe has helpful gene"},"content":{"rendered":"

Rice University discovery could lessen need for costly cleanup of some contaminated groundwater sites<\/em><\/strong><\/span><\/p>\n

\"\"
Pedro Alvarez (Credit: Jeff Fitlow\/Rice University)<\/figcaption><\/figure>\n

HOUSTON — Rice University researchers have discovered a bacteria-borne gene that helps degrade a form of dioxane, a groundwater contaminant and suspected carcinogen. The discovery could be the basis for a much-needed tool to decide how contaminated sites should be treated.<\/span><\/p>\n

Research by the Rice lab of civil and environmental engineer Pedro Alvarez found a novel gene cluster in\u00a0Mycobacterium<\/u><\/a>\u00a0dioxanotrophicus PH-06, a mold-like bacterium capable of using\u00a01,4-dioxane<\/u><\/a>\u00a0as its sole source of carbon and energy. The microbe was\u00a0discovered in 2009<\/u><\/a>\u00a0in the sediment of a dioxane-contaminated river in South Korea.<\/span><\/p>\n

In an\u00a0earlier study<\/u><\/a>, the Rice team decoded the complete genome sequence of the bacterium. While it clearly fed on dioxane, it did not contain a well-studied gene found in another microbe known to initiate dioxane biodegradation.<\/span><\/p>\n

But the whole genome sequence revealed the presence of a novel propane\u00a0mono-oxygenase<\/u><\/a>\u00a0gene cluster<\/u><\/a>\u00a0that expresses an enzyme the researchers said is also likely to initiate dioxane biodegradation. “This is important because it shows that dioxane-degrading genes are more diverse than previously appreciated,” Alvarez said.<\/span><\/p>\n

The new study appears in the American Chemical Society journal\u00a0Environmental Science & Technology Letters<\/u><\/a>.<\/span><\/p>\n

Dioxane has been widely used as a stabilizer for chlorinated solvents and is commonly found as a co-contaminant at thousands of polluted sites, according to the researchers. The chemical is highly soluble in water, easily leaches into groundwater and is resistant to natural biodegradation, Alvarez said.<\/span><\/p>\n

He said the discovery should help environmental engineers find the best way to treat contaminated groundwater.<\/span><\/p>\n

“Current site remediation approaches like ‘pump and treat’ or\u00a0in situ\u00a0chemical oxidation are not suitable for large and dilute dioxane plumes that prevail at thousands of contaminated sites,” he said. “For such plumes,\u00a0monitored natural attenuation<\/u><\/a>\u00a0(MNA) can be the most cost-effective approach.<\/span><\/p>\n

“However, MNA is underutilized at dioxane-impacted sites because we lack reliable analytical tools to support decisions to select it or reject it,” he said.<\/span><\/p>\n

The gene cluster could be employed as a probe to test for the presence of dioxane degraders in groundwater. If degraders are present, the site might be best left to eliminate dioxane naturally, though with continued monitoring. The gene cluster provides a basis for minimizing false negatives when looking for dioxane degraders at contaminated sites, Alvarez said.<\/span><\/p>\n

“There’s an urgent need for these molecular tools from an economic standpoint,” he said. “Our novel gene probe can enlighten judicious MNA selection and\u00a0avoid significant expenses associated with costly and marginally effective remediation alternatives.”<\/span><\/p>\n

Rice graduate student Ya He is lead author of the study. Co-authors are research scientists Jacques Mathieu and M\u00e1rcio Lu\u00eds Busi Da Silva and postdoctoral researchers Yu Yang and Pingfeng Yu. Alvarez is the George R. Brown Professor of Engineering, chair of Rice’s Department of Civil and Environmental Engineering and director of the Rice-based, National Science Foundation-sponsored\u00a0Nanosystems Engineering Research Center for Nanotechnology-Enabled Water Treatment<\/u><\/a>.<\/span><\/p>\n

The Department of Defense’s\u00a0Strategic Environmental Research and Development Program sponsored the research.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Rice University discovery could lessen need for costly cleanup of some contaminated groundwater sites HOUSTON — Rice University researchers have discovered a bacteria-borne gene that helps degrade a form of dioxane, a groundwater contaminant and suspected carcinogen. The discovery could be the basis for a much-needed tool to decide how contaminated sites should be treated. […]<\/p>\n","protected":false},"author":6,"featured_media":13535,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17],"tags":[],"class_list":["post-13534","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-research"],"featured_image_urls":{"full":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",444,668,false],"thumbnail":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3-150x150.jpg",150,150,true],"medium":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3-199x300.jpg",199,300,true],"medium_large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",444,668,false],"large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",444,668,false],"1536x1536":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",444,668,false],"2048x2048":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",444,668,false],"ultp_layout_landscape_large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",444,668,false],"ultp_layout_landscape":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",379,570,false],"ultp_layout_portrait":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",444,668,false],"ultp_layout_square":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",399,600,false],"newspaper-x-single-post":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",326,490,false],"newspaper-x-recent-post-big":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",239,360,false],"newspaper-x-recent-post-list-image":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",43,65,false],"web-stories-poster-portrait":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",444,668,false],"web-stories-publisher-logo":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",64,96,false],"web-stories-thumbnail":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/11\/1106_DIOXANE-1-web-16zitr3.jpg",150,226,false]},"author_info":{"info":["Amrita Tuladhar"]},"category_info":"Research<\/a>","tag_info":"Research","comment_count":"0","_links":{"self":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts\/13534","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/comments?post=13534"}],"version-history":[{"count":0,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts\/13534\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/media\/13535"}],"wp:attachment":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/media?parent=13534"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/categories?post=13534"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/tags?post=13534"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}