{"id":14015,"date":"2017-12-26T08:17:12","date_gmt":"2017-12-26T08:17:12","guid":{"rendered":"https:\/\/www.revoscience.com\/en\/?p=14015"},"modified":"2020-05-27T06:19:00","modified_gmt":"2020-05-27T06:19:00","slug":"new-hope-stopping-understudied-heart-disease-tracks","status":"publish","type":"post","link":"https:\/\/www.revoscience.com\/en\/new-hope-stopping-understudied-heart-disease-tracks\/","title":{"rendered":"New hope for stopping an understudied heart disease in its tracks"},"content":{"rendered":"
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Biomedical engineering professor Kristyn Masters handles samples in her lab, where she and colleagues identified the early stages of a process that may eventually cause aortic stenosis, a severe narrowing of the aortic valve that reduces blood flow to the body and weakens the heart. (Photo by Stephanie Precourt)<\/figcaption><\/figure>\n

The diminutive size of our aortic valve \u2014 just shy of a quarter \u2014 belies its essential role in pushing oxygen-rich blood from the heart into the aorta, our body\u2019s largest vessel, and from there to all other organs. Yet for decades, researchers have focused less on damaged valves than on atherosclerosis, the gradual hardening of the blood vessels themselves.<\/span><\/p>\n

Thanks, in part, to pigs at the University of Wisconsin\u2013Madison\u2019s Arlington Agricultural Research Station, scientists now are catching up on understanding the roots of calcific aortic valve disease (CAVD).<\/span><\/p>\n

\u201cFor a long time, people thought CAVD was just the valvular equivalent of atherosclerosis,\u201d says Kristyn Masters, a professor of biomedical engineering at UW\u2013Madison. \u201cToday, we know that valve cells are quite unique and distinct from the smooth muscle cells in our blood vessels, which explains why some treatments for atherosclerosis, such as statins, don\u2019t work for CAVD, and why the search for drugs has to start from scratch.\u201d<\/span><\/p>\n

A team led by Masters has jumped a longstanding hurdle in that search with a study published today in the journal Proceedings of the National Academy of Sciences. The researchers teased apart, for the first time, the early cascade of events that may eventually cause stenosis, a severe narrowing of the aortic valve that reduces blood flow to body tissues and weakens the heart.<\/span><\/p>\n

The only current treatment for stenosis is valve replacement, which typically requires risky and expensive open-heart surgery.<\/span><\/p>\n

\u201cOur study sheds new light on the differences between atherosclerosis and CAVD, especially in terms of bottleneck events that we can target with drugs,\u201d says Masters, whose work is supported by the National Institutes of Health and the American Heart Association. \u201cWith a better understanding of how the disease progresses from early to later stages, we may eventually be able to stop CAVD in its tracks and avoid valve replacement surgery.\u201d<\/span><\/p>\n

Since the hearts of mice and other small animals are vastly different from the human organ, CAVD research has long been hampered by a lack of good animal models. That\u2019s why the pigs \u2014 specifically those bred to have an overdose of fatty molecules in their arteries \u2014 were an important starting point for the current study.<\/span><\/p>\n

Their valves provided a snapshot of early CAVD that is challenging to capture in humans, showing that it typically begins with the accumulation of certain sugar molecules called glycosaminoglycans (GAGs) in valve tissue. But to examine exactly how this tissue responds to increasing levels of GAGs, the researchers needed a greater amount of valve tissue than living pigs could provide.<\/span><\/p>\n

That prompted them to create a first-of-its-kind platform mimicking hallmarks of early porcine CAVD in a lab dish. Key for this model was the ability to grow valve cells in their native healthy form, an important distinction from many previous studies that had focused on already diseased cells.<\/span><\/p>\n

When the researchers changed only the amount of GAGs these native valve cells were exposed to, while keeping all other conditions the same, they observed surprising results that challenged previous assumptions.<\/span><\/p>\n

\u201cWe thought the GAGs would play a major role in driving the disease process, but the more we added, the fewer inflammatory factors the cells produced and the happier they were,\u201d says Masters. \u201cWhen we examined this unexpected finding more closely, we noticed two distinct effects: GAGs directly increased a chemical needed to grow new blood vessels, and also trapped low-density lipoprotein (LDL) molecules.\u201d<\/span><\/p>\n

Neither of these effects was immediately detrimental for valve cells, but the trapping made it more likely for oxygen to react with LDL molecules, and the accumulation of oxidized LDL appeared to be a bottleneck event for a multi-stage process toward valve cell damage, Masters says.<\/span><\/p>\n

This multi-stage process may explain why 25 percent of adults over the age of 65 have CAVD with partially blocked aortic valves, but only one percent go on to develop stenosis due to a valve that can no longer open and close properly. The fact that native valve cells cannot oxidize LDL themselves, while smooth muscle cells in blood vessels can, also highlights a key distinction between CAVD and atherosclerosis.<\/span><\/p>\n

The study, which included first author Ana Porras, a postdoctoral in Masters\u2019 lab, has important implications for the development of new drugs that may prevent early CAVD from progressing to stenosis by making GAGs less likely to bind LDL.<\/span><\/p>\n

\u201cThe take-home message of our study is that CAVD is a multi-stage process and that healthy valve cells respond differently to LDL than blood vessel cells,\u201d Masters says. \u201cThe ability to examine multiple steps in this novel in vitro model for early CAVD opens up several promising avenues for developing drugs that are distinct from those for atherosclerosis.\u201d<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

The diminutive size of our aortic valve \u2014 just shy of a quarter \u2014 belies its essential role in pushing oxygen-rich blood from the heart into the aorta, our body\u2019s largest vessel, and from there to all other organs. Yet for decades, researchers have focused less on damaged valves than on atherosclerosis, the gradual hardening […]<\/p>\n","protected":false},"author":6,"featured_media":14016,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[16,17],"tags":[],"class_list":["post-14015","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-biology","category-research"],"featured_image_urls":{"full":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",775,606,false],"thumbnail":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606-150x150.jpg",150,150,true],"medium":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606-300x235.jpg",300,235,true],"medium_large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606-768x601.jpg",750,587,true],"large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",750,586,false],"1536x1536":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",775,606,false],"2048x2048":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",775,606,false],"ultp_layout_landscape_large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",775,606,false],"ultp_layout_landscape":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",729,570,false],"ultp_layout_portrait":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",600,469,false],"ultp_layout_square":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",600,469,false],"newspaper-x-single-post":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",627,490,false],"newspaper-x-recent-post-big":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",460,360,false],"newspaper-x-recent-post-list-image":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",83,65,false],"web-stories-poster-portrait":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",640,500,false],"web-stories-publisher-logo":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",96,75,false],"web-stories-thumbnail":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2017\/12\/masters-775x606.jpg",150,117,false]},"author_info":{"info":["Amrita Tuladhar"]},"category_info":"Biology<\/a> Research<\/a>","tag_info":"Research","comment_count":"0","_links":{"self":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts\/14015","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/comments?post=14015"}],"version-history":[{"count":0,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts\/14015\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/media\/14016"}],"wp:attachment":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/media?parent=14015"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/categories?post=14015"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/tags?post=14015"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}