{"id":18913,"date":"2020-08-14T12:17:54","date_gmt":"2020-08-14T06:32:54","guid":{"rendered":"https:\/\/www.revoscience.com\/en\/?p=18913"},"modified":"2020-08-14T12:18:01","modified_gmt":"2020-08-14T06:33:01","slug":"tracking-spreads-covid-19-through-untreated-wastewater","status":"publish","type":"post","link":"https:\/\/www.revoscience.com\/en\/tracking-spreads-covid-19-through-untreated-wastewater\/","title":{"rendered":"Tracking spreads COVID-19 through untreated wastewater"},"content":{"rendered":"\n
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Researchers track a spreads COVID-19 through untreated wastewater, which is quick\u2013cost-effective approach.<\/p>\n\n\n\n

To test a virus in untreated wastewater, they have used seven methods\u2014adsorption-extraction technique can most efficiently detect SARS-CoV-2. This gives us another tool to detect the presence and spread of the COVID-19 pandemic, researcher said.<\/p>\n\n\n\n

In the first step, they were tracking the spread of the COVID-19 pandemic is currently conducted by testing nasal swabs or saliva samples. This is not a new technique, and has been used for detecting non-enveloped viruses, but a conventional method for enveloped viruses such as SARS-CoV-2 had not been developed.<\/p>\n\n\n\n

Assistant Professor Masaaki Kitajima from the Water Quality Control Engineering Laboratory at Hokkaido University, scientists has reported a fast, economical method to concentrate coronavirus in untreated wastewater.<\/p>\n\n\n\n

Murine hepatitis virus (MHV), a type of enveloped virus, is closely related to SARS-CoV-2 but does not affect humans, and is thus safe to use for testing the feasibility of the method. The study was published in Science of the Total Environment.<\/p>\n\n\n\n

The scientists obtained MHV from mice feces and introduced it into samples of untreated wastewater collected from Brisbane, Australia.<\/p>\n\n\n\n

They attempted to recover and concentrate the MHV from these samples by seven different methods which are commonly used to test for non-enveloped viruses.<\/p>\n\n\n\n

The amount of recovered MHV was determined by a method called reverse transcription-quantitative PCR, where the RNA of the virus extracted, converted to DNA, the DNA is repeatedly duplicated, and the increase in amount of DNA is measured throughout the process.<\/p>\n\n\n\n

The recovery was highest in the method that involved treating the sample with magnesium chloride and then filtering out the virus on a negatively-charged membrane; the second highest recovery was by a similar method without magnesium chloride.<\/p>\n\n\n\n

The advantages of these methods include an initial processing time of under 1 hour and the need only for cheap, widely available equipment and reagents.<\/p>\n\n\n\n

There are also drawbacks, such as the clogging of the filters that may increase processing time.<\/p>\n\n\n\n

However, to date, the need for reverse transcription-qPCR for the detection of the virus is unavoidable.<\/p>\n\n\n\n

The next step would be to test this method in samples collected from areas where the pandemic is prevalent.<\/p>\n\n\n\n

There are two objectives: one is to show that the technique can be used for SARS-CoV-2, and the other is to show that the test can be used on samples from outside the lab.<\/p>\n\n\n\n

\u201cI hope this research contributes to the establishment of a standard protocol for the detection of SARS-CoV-2 in wastewater,\u201d says Assistant Professor Kitajima, \u201cand this, in turn, accelerates investigations to enhance our understanding of COVID-19 epidemiology through wastewater surveillance.\u201d<\/p>\n","protected":false},"excerpt":{"rendered":"

Researchers track a spreads COVID-19 through untreated wastewater, which is quick\u2013cost-effective approach.<\/p>\n","protected":false},"author":2,"featured_media":18914,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[16,6],"tags":[],"class_list":["post-18913","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-biology","category-health"],"featured_image_urls":{"full":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method.png",3000,2100,false],"thumbnail":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method-200x200.png",200,200,true],"medium":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method-300x210.png",300,210,true],"medium_large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method-768x538.png",750,525,true],"large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method-1024x717.png",750,525,true],"1536x1536":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method-1536x1075.png",1536,1075,true],"2048x2048":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method-2048x1434.png",2048,1434,true],"ultp_layout_landscape_large":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method.png",1143,800,false],"ultp_layout_landscape":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method.png",814,570,false],"ultp_layout_portrait":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method.png",600,420,false],"ultp_layout_square":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method.png",600,420,false],"newspaper-x-single-post":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method-760x490.png",760,490,true],"newspaper-x-recent-post-big":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method-550x360.png",550,360,true],"newspaper-x-recent-post-list-image":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method-95x65.png",95,65,true],"web-stories-poster-portrait":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method.png",640,448,false],"web-stories-publisher-logo":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method.png",96,67,false],"web-stories-thumbnail":["https:\/\/www.revoscience.com\/en\/wp-content\/uploads\/2020\/08\/recovery-method.png",150,105,false]},"author_info":{"info":["RevoScience"]},"category_info":"Biology<\/a> Health<\/a>","tag_info":"Health","comment_count":"0","_links":{"self":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts\/18913","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/comments?post=18913"}],"version-history":[{"count":0,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/posts\/18913\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/media\/18914"}],"wp:attachment":[{"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/media?parent=18913"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/categories?post=18913"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.revoscience.com\/en\/wp-json\/wp\/v2\/tags?post=18913"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}